MLB's Rays honour Orlando shooting victims

The Tampa Bay Rays have honoured victims of Orlando's mass shooting with a dedicated Pride Night, which drew their largest regular season crowd in more than 10 years.

Forty-nine people were killed at an Orlando night club on Sunday in what was the deadliest mass shooting in modern US history.

The Rays chose to make their contest against the San Francisco Giants on Friday a night of observance as Tropicana Field in St Petersburg hosted 40,135 fans, the most at the ballpark since opening day in 2006.

The team wore "We are Orlando" T-Shirts for batting practice and hats of their former minor league team, the Orlando Rays, during the contest.

A tribute video from both teams played on the scoreboard.

Tampa Bay also made tickets for the game available for just $US5 ($A6.80) beginning on Tuesday and donated the proceeds to a victim's fund.

The Rays announced they raised more than $US300,000.

"Orlando is a huge part of baseball's heart," Major League Baseball's vice-president of social responsibility Billy Bean said.

"What happened in Orlando happened to everybody and I've been very proud of the way baseball has responded with wanting to be supportive."

The Giants beat the Rays 5-1.